Proteomic analysis of arsenic-induced PML body associated proteins. (A) Overview of the proteomic experimental design. For each condition, n = 4 separate experiments. (B) Scatter plot of log₂ ratios comparing protein abundance in anti-GFP nanobody purifications from YFP-PML expressing PML^−/− U2OS cells with those from WT U2OS cells (x-axis) and comparing abundance in purifications form YFP-PML cells during 1 μM arsenic treatment with the same purifications from cells not exposed to arsenic (y-axis). Data for 2,157 proteins shown. Red markers are proteins defined as significantly different in both comparisons (see Fig. S2). (C) Summary data for the 21 proteins marked red in B. Underlined gene names were identified previously as PML proximal proteins (Barroso-Gomila et al., 2021), and bold entries are described in the Human Protein Atlas (Thul et al., 2017; http://proteinatlas.org) as belonging to PML body. (D) Anti-GFP nanobody purifications were prepared from the indicated cells after 6 h 1 μM arsenic (+As) or untreated (−As). Both inputs and bead elutions were Western blotted for the indicated species. Molecular weight markers are indicated in kD. Source data are available for this figure: SourceData F1.