Automated screen on COPB1 cells in light and electron microscopy 48 h after liquid phase transfection knockdown. (a) Overview of 25 selected cells in a screen for COPB1 knockdown. Light microscopy images (green is GFP GalNAc-T2 Golgi apparatus and blue is DAPI for the nucleus, top) and the corresponding electron microscopy images (bottom). (b) Top: Selected control cell (treated with XWNeg9 siRNA) in light microscopy (left), electron microscopy (middle), and a reconstructed model from the FIB-SEM stack (right) showing the 3D model of the nucleus in blue, the model of the Golgi stacks in green and a surface rendering of the cell surface in transparent green. Bottom: Selected COPB1 cell (treated with COPB1 siRNA) in light microscopy (left), electron microscopy (middle), and a reconstructed model (right). (c) Detailed electron microscopy images of the Golgi apparatus region in a control cell (left) and four different variations of a disturbed Golgi apparatus in different selected cells of the COPB1 knockdown. Scale bars: (a) LM—10 µm, EM—5 µm, (b) left to right—10, 2, 5 µm, (c) 1 µm.