Figure S3.
Comparison of the fluorescent intensity of CoA-PEG11-NR linked to 1992-ACP-IR, 2031-ACP-IR, or PreCT-ACP-IP. Top: Images of double staining of PreCT-ACP-IR, 1992-ACP-IR, or 2031-ACP-IR stably expressing cells with CoA-PEG11-NR (left), and with anti-insulin receptor mouse monoclonal antibody and Cy2-conjugated anti mouse secondary antibody (right). Small circles on the plasma membrane are ROIs for measurement of the fluorescent intensity of Cy2 and Nile Red. Bottom: The fluorescent ratio of Nile Red and Cy2 was calculated for PreCT-ACP-IR (n = 169 ROIs), 1992-ACP-IR (n = 53) and 2031-ACP-IR (n = 171). Data represent the mean ± SEM. Statistical significance was calculated by one-way ANOVA, ns, not significant, and ****, P < 0.0001.

Comparison of the fluorescent intensity of CoA-PEG11-NR linked to 1992-ACP-IR, 2031-ACP-IR, or PreCT-ACP-IP. Top: Images of double staining of PreCT-ACP-IR, 1992-ACP-IR, or 2031-ACP-IR stably expressing cells with CoA-PEG11-NR (left), and with anti-insulin receptor mouse monoclonal antibody and Cy2-conjugated anti mouse secondary antibody (right). Small circles on the plasma membrane are ROIs for measurement of the fluorescent intensity of Cy2 and Nile Red. Bottom: The fluorescent ratio of Nile Red and Cy2 was calculated for PreCT-ACP-IR (n = 169 ROIs), 1992-ACP-IR (n = 53) and 2031-ACP-IR (n = 171). Data represent the mean ± SEM. Statistical significance was calculated by one-way ANOVA, ns, not significant, and ****, P < 0.0001.

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