ZNCC images reveal that the local membrane environment of the insulin receptor is dynamic, and changes are heterogeneous. (A) A representative time-lapse data set of pseudocolor ZNCC images filtering the median filter (3 × 3 pixels). Arrowheads show areas intensely blebbing and membrane ruffling. Scale bar, 10 μm. The whole time-lapse image used for making the kymographs set is shown in Fig. S2. (B) Kymographs of chosen areas show time-course ZNCC changes. The horizontal dimension of the kymograph represents the vertically averaged ZNCC inside the rectangular area, and the vertical dimension of the kymograph represents the time (from t = 1 to t = 55) change of its averaged ZNCC. Scale bar, 10 μm.