GP images of the local membrane environment surrounding the insulin receptor. A representative time-lapse data set of pseudocolor GP images filtering the median filter (3 × 3 pixels). The pseudocolor GP images are depicted by logarithmic scale from 10⁻¹–10⁰. The GP value <10⁻¹ is displayed in the same color as 10⁻¹; scale bar, 10 μm. This time-lapse image set was one data set utilized for delta GP change compared to time 0 in Fig. 4 B.