Comparison of emission spectra of CoA-PEG5-NR linked to 2031-ACP-IR in the time-course. Emission spectrum of a representative time-course observation of CoA-PEG5-NR linked to 2031-ACP-IR (Fig. 3 C) at time 0 s, at 230 s, before insulin treatment, and at 540 s, the last time point.