Temporal reconstruction of clathrin coat remodeling across three different cell lines. (A and B ) Superresolution averages for distinct endocytic stages, resulting from all collected snapshots for (A) U2OS (n = 241 sites, N = 3 cells) and (B) 3T3 (n = 688 sites, N = 7 cells). Each bin contains the same number of snapshots of clathrin-coated structures sorted along their pseudotime (n_U2OS = 24 per bin, n_3T3 = 68) and represent equally long pseudotime intervals.