Clathrin coat remodeling in three different cell lines. (A–C) Results of LocMoFit analysis for clathrin structures. Different growth models are fitted to curvature H over θ. The resulting fitting parameters are then used to map the same models also over the surface area, edge length, and projected area (left to right). Purple: Completely flat sites with H = 0 nm⁻¹ and θ = 0. Red: Disconnected sites that were excluded from the fitting. Black line: Rolling median (window width = 5% of total number of sites; A) U2OS (N = 3 cell, n_grey = 241 sites, n_red = 53 disconnected sites, n_purple = 1 completely flat sites), (B) 3T3 mouse fibroblasts (N = 7 cells, n_grey = 688 sites, n_red = 51 disconnected sites, n_purple = 8 completely flat sites), and (C) SK-MEL-2 cells (N = 13 cells, n_grey = 1,631 sites, n_red = 153 disconnected sites, n_purple = 14 completely flat sites). (D–F) Temporal reconstruction of clathrin coat remodeling. (i) Distribution of θ slightly differs between cell lines, especially in the earlier states. Median θ shown as dotted lines correspond to 99.6° for U2OS; 121.4° for 3T3; and 108.5° for SK-MEL-2 cells. (ii) The cooperative curvature model (red line) highlights the square-root dependence between θ and pseudotime. (iii) The cooperative curvature model is used to describe the curvature H propagation over pseudotime. Resulting fitting parameters are then used to map the same model to surface area A, edge length Ɛ, and projected area A_p. A rolling median is plotted in black (window width = 5% of total number of sites).