Cytoskeletal structures in pupal dendrites and epidermis. (A and B) Effect of CCAP neuron ablation on dendritic microtubule disassembly at 6 h APF in lateral c4da neurons (v’ada). v’ada neurons were visualized with ppk-CD4::tdtomato, and microtubules were stained with anti-futsch (22C10) antibodies. A′ and B′ show v’ada dendrite regions marked in A and B; A″ and B″ show the corresponding futsch/22C10 stainings. (A–A″) v’ada in control animal expressing GFP in CCAP neurons. (B–B″) v’ada in animal with ablated CCAP neurons. (C–E) Epidermal actin structures surround both severed and non-severed c4da neuron dendrites. C4da neurons were visualized with ppk-CD4::tdtomato at 9 h APF. (C′–E′) show the F-actin reporter UAS-lifeact::GFP under control of the epidermal driver A58-GAL4. (C″–E″) Merge. (C–C″) Example of epidermal actin encasing a severed c4da neuron dendrite. (D–D″ and E–E″) Examples of epidermal actin encasing c4da neuron dendrites still attached to the cell body. The arrow in C indicates a severing site, arrows in D and E indicate the position of the cell body. Asterisks in C′–E′ and C″–E″ indicate close apposition between dendrites and epidermal actin. Scale bars are 10 μm in A, A′, and C and 20 μm in D and E.