Ecdysis/head eversion defects upon loss of E74 and upon CCAP neuron ablation. Animals of the indicated genotypes were dissected out of the pupal case at 18 h APF and photographed from the top (upper panels) and side (lower panels). Note smaller heads, lack of legs, and longer abdomens in experimental genotypes, indicative of ecdysis defects. (A) Control animal heterozygous for E74^DL-1. (B) E74^DL-1/v4 mutant animal. (C) Control animal expressing GFP in CCAP neurons. (D) Animal with ablated CCAP neurons (hid expression under CCAP-GAL4). (E–G) The GAL4 line act-GAL4, ppk-GAL80/CyOweeP; ppk-eGFP (III) was crossed to UAS-mCD8::RFP and dorsal cluster sensory neurons were imaged at the third instar larval stage. A single confocal slice is shown for clarity. (E) C4da neuron labeled by ppk-eGFP. (F) Dorsal cluster da neurons labeled byCD8::RFP expression under act-GAL4. (G) Merge. Positions of da neuron cell bodies are indicated by asterisks. (H and I) Pupae ubiquitously expressing the indicated dsRNAs with exception of c4da neurons (act-GAL4, ppk-GAL80). (H) Control animal ubiquitously expressing Orco dsRNA. (I) Animal expressing E74 dsRNA. Scale bars are 1 mm in A–D, H, and I and 10 μm in E.