Morphogenetic movement of c4da neuron cell bodies after 8 h APF is linked to pupal ecdysis. (A–C) c4da neuron cell body tracings in time lapse movies (5-min intervals) of animals of the indicated genotypes between 8 and 10 h APF. (A) Heterozygous E74^DL-1/+ control. (B)E74^DL-1/v4 mutant. (C) Animal with ablated CCAP neurons (hid expression under CCAP-GAL4). (D) Graph depicting amplitude of c4da neuron cell body movements of the genotypes in B–D during the early pupal stage. Traces show average displacement from previous position in μm (N = 8–10 neurons each) over time (h APF). (E–F′) Spatiotemporal image correlation analysis of tissue movement. Arrows depict velocity of local tissue movement as determined by tracing epidermal mitochondria in the vicinity of the c4da neuron (see color code for movement velocity) in 1-s intervals in single confocal planes. E and E′ show representative frames of a c4da neuron at 7.5 h APF; F and F′ show representative frames of a c4da neuron at 10 h APF.