Timecourse analysis of PNS neuron dendrite severing. (A–F) Representative images of dorsal ddaC c4da neurons (visualized by ppk-CD4::tdtomato) at the indicated developmental timepoints. (G) Graph depicting the average number of primary dendrites attached to the c4da neuron cell body at the indicated timepoints. Values are mean ± SD, *P < 0.05, ***P < 0.0005, Mann–Whitney U test, N = 22–63 neurons per timepoint. (H–M) Representative images of v'ada c4da neurons at the indicated developmental timepoints. (N) Graph depicting the average number of primary dendrites attached to the v'ada neuron cell body at the indicated timepoints. Values are mean ± SD, *P < 0.05, ***P < 0.0005, Mann–Whitney U test. N = 22–30 neurons per timepoint. (O–T) Timecourse of dendrite severing during dendrite pruning of dorsal c1da neurons ddaD (right) and ddaE (left; visualized by expression of UAS-tdtomato under the control of GAL4^2-21). Panels show representative images of c1da neurons at the indicated developmental timepoints. (U) Graph depicting the average number of primary and secondary dendrites attached to the cell body of ddaE and ddaD at the indicated timepoints. Values are mean ± SD, **P < 0.005, ***P < 0.0005, Wilcoxon test. N = 9–14 neurons per timepoint. Blue arrowheads in panels indicate axons. Scale bars are 50 μm in A and 100 μm in H and O.