Related to Fig. 1 . Increased Par-1 levels and active site phosphorylation in response to ecdysone. S2R+ cells transfected with an empty control plasmid or expression plasmids for FLAG-tagged wild type Par-1 or the non-phosphorylatable mutant Par-1 T636A were treated with 20-hydroxy-ecdysone (20E) for 3 h, lysed and analyzed by Western blot with antibodies against the phosphorylated Par-1 activation loop (phospho-MARK), FLAG or VCP as a loading control (lower blot). Running positions of molecular weight markers are indicated on the right. Source data are available for this figure: SourceData FS1.