Virus-mediated hPLS3overexpression in L1 spinal segment motoneurons. Motoneuron soma within the L1 lumbar spinal segment of P10 control animals injected with AAV9-EGFP or AAV9-hPLS3, stained against choline acetyltransferase (ChAT, red), HA (gray) and GFP (green), scale bar: 50 µm. Quantification of the percentage of GFP (AAV9-EGFP) or HA (AAV9-hPLS3)-expressing motoneurons (n = 3/4). Data are presented as mean ± SD.