Figure 2.
Actin-dependent cAMP-induced TrkB surface recruitment is impaired in Smn-deficient motoneuron growth cones. (A–C) Growth cones from control and Smn−/−;SMN2 motoneurons stained against surface TrkB (gray) and Synaptophysin-1 (SYP, magenta). Scale bars: 5 µm. (A) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals unstimulated and stimulated for 5 min and 15 min BDNF (N = 3, n = 93; ANOVA Kruskal–Wallis, **P ≤ 0.01; ****P ≤ 0.0001). (B) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals unstimulated and 20 min 8-CPT-cAMP stimulated (N = 4, n = 125; ANOVA Kruskal–Wallis, ****P ≤ 0.0001). (C) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals treated with 0.5 µM Cytochalasin D (CytoD) prior to 20 min 8-CPT-cAMP stimulation (N = 3, n = 90; ANOVA Kruskal–Wallis, ****P ≤ 0.0001). Data are presented as scatter dot plot with bar. Bars represent the mean ± SEM.

Actin-dependent cAMP-induced TrkB surface recruitment is impaired in Smn-deficient motoneuron growth cones. (A–C) Growth cones from control and Smn−/−;SMN2 motoneurons stained against surface TrkB (gray) and Synaptophysin-1 (SYP, magenta). Scale bars: 5 µm. (A) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals unstimulated and stimulated for 5 min and 15 min BDNF (N = 3, n = 93; ANOVA Kruskal–Wallis, **P ≤ 0.01; ****P ≤ 0.0001). (B) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals unstimulated and 20 min 8-CPT-cAMP stimulated (N = 4, n = 125; ANOVA Kruskal–Wallis, ****P ≤ 0.0001). (C) Normalized mean gray values of surface TrkB in control and Smn−/−;SMN2 terminals treated with 0.5 µM Cytochalasin D (CytoD) prior to 20 min 8-CPT-cAMP stimulation (N = 3, n = 90; ANOVA Kruskal–Wallis, ****P ≤ 0.0001). Data are presented as scatter dot plot with bar. Bars represent the mean ± SEM.

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