Figure S5.

Active Atg15 degrades phospholipids in membrane. (A) Lipase activity of vacuolar lysates toward 400-nm-diameter liposomes containing NBD-PE. The incubation time was 2 h. TLC analysis (left panels) shows representative results from three independent experiments. The bar graphs (right panels) represent the relative intensity calculated as described in Fig. 1 C. Error bars represent means ± SD of three independent experiments. (B) Lipase activity of vacuolar lysates toward 100-nm-diameter liposomes containing NBD-PE. The incubation time was 2 h. TLC analysis (left panels) shows representative results from three independent experiments. The bar graphs (right panels) represent the relative intensity calculated as described in Fig. 1 C. Error bars represent means ± SD of three independent experiments. Source data are available for this figure: SourceData FS5.

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