Vacuolar lysate has lipase activity derived from Atg15 of broad specificity. (A and B) Lipase activity of vacuolar lysates from atg15Δ cells expressing Atg15^iFLAG and Atg15^iFLAG,S332A. The lysates were incubated with NBD-PE, NBD-PS, NBD-PC, and NBD-PG, each labeled at sn-2 (A), and NBD-LPC (B) for 1 h. (C) Lipase activity of vacuolar lysates toward TF-PI labeled at sn-2. The incubation time was 1 h. (D) Lipase activity of vacuolar lysates toward TF-TG and BODIPY-CE. The incubation time was 2 h. Source data are available for this figure: SourceData FS4.