Figure 3.
The vacuolar luminal region of Atg15 binds to the AB membrane. Spinning-disk confocal microscopy image of pep4Δ prb1Δ cells expressing Atg15-mNG or CPYN50-Atg15ΔN35-mNG. Cells were grown in YPD medium and treated with rapamycin for 3 h. ABs are indicated by mCherry-Atg8 fluorescence. The green and pink arrowheads indicate ER and aggregated Atg15-mNG, respectively. Dashed lines indicate vacuole (yellow) and cell (cyan) boundaries. Scale bar, 1 µm. See also Videos 2 and 3.

The vacuolar luminal region of Atg15 binds to the AB membrane. Spinning-disk confocal microscopy image of pep4Δ prb1Δ cells expressing Atg15-mNG or CPYN50-Atg15ΔN35-mNG. Cells were grown in YPD medium and treated with rapamycin for 3 h. ABs are indicated by mCherry-Atg8 fluorescence. The green and pink arrowheads indicate ER and aggregated Atg15-mNG, respectively. Dashed lines indicate vacuole (yellow) and cell (cyan) boundaries. Scale bar, 1 µm. See also Videos 2 and 3.

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