Figure S5.
CCDC15 and inner scaffold proteins depend on each other for centrosomal abundance. (A–E) Representative images and quantification of effect of CCDC15 depletion on centrosomal levels of (A) POC1B and Centrin-2 (for POC1B, siControl = 1 ± 0.45 siCCDC15 = 0.62 ± 0.35, P < 0.0001; and for Centrin-2, siControl = 1 ± 0.38, siCCDC15 = 0.97 ± 0.35, P = 0.2083); (B) POC5 (siControl = 1 ± 0.01, siCCDC15 = 1.15 ± 0.06, P < 0.0001); (C) FAM161A (siControl = 1 ± 0.57, siCCDC15 = 0.97 ± 0.58, P = 0.6618); (D) CEP63 (siControl = 1 ± 0.23, siCCDC15 = 0.96 ± 0.17, P = 0.2143); and (E) CEP164 (siControl = 1 ± 0.14, siCCDC15 = 0.98 ± 0.16, P = 0.5910). RPE1 cells were fixed 96 h after transfection with control or CCDC15 siRNA and stained for the indicated proteins. DNA was visualized with DAPI. Error bars, SD. n > 100 cells per experiment. Data represent mean value from three experiments per condition. Two-sided t test. Scale bar, 1 μm, insets, 0.5 μm. (F) Representative images of the effect of POC5, POC1B, or FAM161A depletion on centrosomal levels of CCDC15. RPE1 cells were fixed 96 h after transfection with the indicated siRNAs and stained for CCDC15, Cenrin-2, and γ-tubulin. DNA was visualized with DAPI. Scale bar, 5 μm. (G) Quantification of CCDC15 centrosomal intensity based on F. Error bars, SD. n > 100 cells per experiment. Data represent mean value from three experiments per condition. siControl = 1 ± 0.4; siPOC1B = 0.44 ± 0.32; siPOC5 = 1.3 ± 0.53; siFAM161A = 0.64 ± 0.24, P < 0.0001, two-sided t test. **** P < 0.0001, ns: non-significant.

CCDC15 and inner scaffold proteins depend on each other for centrosomal abundance. (A–E) Representative images and quantification of effect of CCDC15 depletion on centrosomal levels of (A) POC1B and Centrin-2 (for POC1B, siControl = 1 ± 0.45 siCCDC15 = 0.62 ± 0.35, P < 0.0001; and for Centrin-2, siControl = 1 ± 0.38, siCCDC15 = 0.97 ± 0.35, P = 0.2083); (B) POC5 (siControl = 1 ± 0.01, siCCDC15 = 1.15 ± 0.06, P < 0.0001); (C) FAM161A (siControl = 1 ± 0.57, siCCDC15 = 0.97 ± 0.58, P = 0.6618); (D) CEP63 (siControl = 1 ± 0.23, siCCDC15 = 0.96 ± 0.17, P = 0.2143); and (E) CEP164 (siControl = 1 ± 0.14, siCCDC15 = 0.98 ± 0.16, P = 0.5910). RPE1 cells were fixed 96 h after transfection with control or CCDC15 siRNA and stained for the indicated proteins. DNA was visualized with DAPI. Error bars, SD. n > 100 cells per experiment. Data represent mean value from three experiments per condition. Two-sided t test. Scale bar, 1 μm, insets, 0.5 μm. (F) Representative images of the effect of POC5, POC1B, or FAM161A depletion on centrosomal levels of CCDC15. RPE1 cells were fixed 96 h after transfection with the indicated siRNAs and stained for CCDC15, Cenrin-2, and γ-tubulin. DNA was visualized with DAPI. Scale bar, 5 μm. (G) Quantification of CCDC15 centrosomal intensity based on F. Error bars, SD. n > 100 cells per experiment. Data represent mean value from three experiments per condition. siControl = 1 ± 0.4; siPOC1B = 0.44 ± 0.32; siPOC5 = 1.3 ± 0.53; siFAM161A = 0.64 ± 0.24, P < 0.0001, two-sided t test. **** P < 0.0001, ns: non-significant.

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