Glycolytic payoff phase enzymes are located at the growth cone during axonal retraction. (A) Time-lapse images of a hippocampal growth cone and adjacent axonal shaft expressing Lifeact-mCherry (magenta) and GAPDH-eGFP (green) during growth and 100 nM WIN–induced axonal retraction (related to Video 1). (B) Confocal images of hippocampal neurons immunostained for endogenous GAPDH (green) and phalloidin (magenta). Below is shown an enlargement of the growth cone with the orthogonal views. (C) Immunofluorescence images of hippocampal neurons, and enlarged images in growth conditions (control, DMSO) or after 2-min-induced retraction by 0.2 U/ml calpeptin. F-actin staining with phalloidin shown in magenta; microtubules detected with ßIII-tubulin shown in blue, and NMII, GAPDH, and PGK shown in yellow. Growth cones are recognizable by their characteristic morphology with the phalloidin staining in magenta.