The five glycolytic payoff phase enzymes are located at the growth cone and near the shaft during axonal retraction. (Related to Fig. 3.) Schematic representation of the steps of glycolysis split into investment and payoff phases. In green, immunostaining of growth cones for each of the five glycolytic enzymes of the payoff phase in hippocampal cultures treated for 5 min with control (DMSO) or 0.2 U/ml calpeptin. Growth cones are recognized by their characteristic morphology with the phalloidin staining in magenta. Note the collapse of growth cones by calpeptin as compared to control. HK, hexokinase; PGI, glucose-6-phosphate isomerase; PFK, phosphofructokinase; ALDO, aldolase; PGAM, phosphoglycerate mutase; ENO, enolase.