Figure S1.
Growth cone collapse quantification. (Related to Fig. 1.) (A) Left, the shape of the growth cones before and after WIN-induced retraction under metabolic inhibition (oligomycin [oli], 2DG, or control). Center, enlargement of the growth cones with pseudocolor allowing the drawing of the region of interest (ROI, right). (B and C) Bar graphs with ± SEM of ROI calculated area (B and C) circularity. The formula for circularity is 4π (area/perimeter2), and a value of 1.0 indicates a perfect circle (imageJ). (D) Statistical analyses (df: degrees of freedom, F: ANOVA F-value, p: probability of a type I error).