Figure 4.
SHARK and FAK kinases function downstream of SRC in polar cell internalization. (A–G’) Single sections from confocal images of egg chambers at stage 10 expressing UAS-PLCδPHGFP and the genotypes indicated. In A–C, egg chambers were stained with SHARK-Y927 (pSHARK; gray), and in E–G, egg chambers were stained with FAK-Y397 (pFAK; gray). DAPI (blue) was used to visualize DNA. Scale bar is 50 µm. Yellow insets frame border cell clusters, and their respective crop images are shown in A’–G’. (A’’–G’’) Schematic representations of the images shown in A’–G’. White or yellow asterisks indicate non-internalized or partially internalized polar cells, respectively. (A’’’–G’’’) Same images are shown in A’–G’, where pSHARK (A’’’–C’’’) or pFAK (E’’’–G’’’) are shown in Royal LUT, where white represents the highest amount of the protein and black the lowest one. Scale bar is 5 µm. (D–H) Quantifications of normalized pSHARK (D) or pFAK (H) maximal intensity (see Materials and methods) from stage 9 or 10 egg chambers of the indicated genotypes. Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cluster analyzed and their total number per genotype (n) is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were normally distributed, an ANOVA test (one-tailed) with post-hoc Tukey was performed to evaluate statistical significance. P values are shown at the top of the graph. ns indicates not significant. (I) Quantifications of polar cells internalization from egg chambers at stage 10 of the indicated genotypes (see Materials and methods). Error bars: mean ± SEM (n = 3). The total number of clusters analyzed (n) is indicated at the top of each bar. Statistical significance was assessed with a Fisher exact test (one-tailed) and P values are shown at the top of the graph.

SHARK and FAK kinases function downstream of SRC in polar cell internalization. (A–G’) Single sections from confocal images of egg chambers at stage 10 expressing UAS-PLCδPHGFP and the genotypes indicated. In A–C, egg chambers were stained with SHARK-Y927 (pSHARK; gray), and in E–G, egg chambers were stained with FAK-Y397 (pFAK; gray). DAPI (blue) was used to visualize DNA. Scale bar is 50 µm. Yellow insets frame border cell clusters, and their respective crop images are shown in A’–G’. (A’’–G’’) Schematic representations of the images shown in A’–G’. White or yellow asterisks indicate non-internalized or partially internalized polar cells, respectively. (A’’’–G’’’) Same images are shown in A’–G’, where pSHARK (A’’’–C’’’) or pFAK (E’’’–G’’’) are shown in Royal LUT, where white represents the highest amount of the protein and black the lowest one. Scale bar is 5 µm. (D–H) Quantifications of normalized pSHARK (D) or pFAK (H) maximal intensity (see Materials and methods) from stage 9 or 10 egg chambers of the indicated genotypes. Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cluster analyzed and their total number per genotype (n) is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were normally distributed, an ANOVA test (one-tailed) with post-hoc Tukey was performed to evaluate statistical significance. P values are shown at the top of the graph. ns indicates not significant. (I) Quantifications of polar cells internalization from egg chambers at stage 10 of the indicated genotypes (see Materials and methods). Error bars: mean ± SEM (n = 3). The total number of clusters analyzed (n) is indicated at the top of each bar. Statistical significance was assessed with a Fisher exact test (one-tailed) and P values are shown at the top of the graph.

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