Figure S2.
Rac, Rho, SHARK, and FAK are required for activated SRC to affect border cell migration and morphology. (A) Quantifications of normalized pSRC maximal intensity from egg chambers in stage 9 or 10 of the indicated genotypes (see Materials and methods). Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cluster analyzed and their total number per genotype (n) is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were normally distributed, an ANOVA test (one-tailed) with post-hoc Tukey was performed to evaluate statistical significance. P values are shown at the top of the graph. (B–C’) Maximum intensity projection from three slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and UAS-SRC-WT. Egg chambers were stained with pSRC (gray) and FAS3 (magenta) in B and B’, and with F-actin (magenta) in C and C’. DNA is visualized by DAPI (blue). White asterisks in B and B’ indicate non-internalized polar cells and yellow ones in B–C’ indicate partially internalized polar cells. White arrows in B’ point to pSRC accumulation, and yellow arrows in C’ point to F-actin accumulation. Scale bar: 5 µm. (D and E) Quantification of border cell migration in stage 10 egg chambers for the genotypes indicated (see Materials and methods). The total number of samples is indicated on top of each bar. (F–H’) Single sections of border cell clusters from stage 10 egg chambers expressing the genotypes indicated. Egg chambers were stained with CAD (green) and FAS3 (magenta). Yellow asterisks in F indicate internalized polar cells and white ones in G and H indicate non-internalized polar cells. Polar cells are outlined with a yellow dashed line. Scale bar: 5 µm.

Rac, Rho, SHARK, and FAK are required for activated SRC to affect border cell migration and morphology. (A) Quantifications of normalized pSRC maximal intensity from egg chambers in stage 9 or 10 of the indicated genotypes (see Materials and methods). Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cluster analyzed and their total number per genotype (n) is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were normally distributed, an ANOVA test (one-tailed) with post-hoc Tukey was performed to evaluate statistical significance. P values are shown at the top of the graph. (B–C’) Maximum intensity projection from three slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and UAS-SRC-WT. Egg chambers were stained with pSRC (gray) and FAS3 (magenta) in B and B’, and with F-actin (magenta) in C and C’. DNA is visualized by DAPI (blue). White asterisks in B and B’ indicate non-internalized polar cells and yellow ones in B–C’ indicate partially internalized polar cells. White arrows in B’ point to pSRC accumulation, and yellow arrows in C’ point to F-actin accumulation. Scale bar: 5 µm. (D and E) Quantification of border cell migration in stage 10 egg chambers for the genotypes indicated (see Materials and methods). The total number of samples is indicated on top of each bar. (F–H’) Single sections of border cell clusters from stage 10 egg chambers expressing the genotypes indicated. Egg chambers were stained with CAD (green) and FAS3 (magenta). Yellow asterisks in F indicate internalized polar cells and white ones in G and H indicate non-internalized polar cells. Polar cells are outlined with a yellow dashed line. Scale bar: 5 µm.

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