Figure 2.
SRC hyperactivity disrupts border cell morphology and cluster cohesion. (A) Quantifications of the circularity index of individual border cells for the genotypes indicated. Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cell analyzed, and the total number per genotype is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were not normally distributed, a Mann–Whitney U test (one-tailed) was performed between controls and each experimental condition. P values are shown at the top of the graph. (B–F) Maximum intensity projection from eight slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and the genotypes indicated. White, blue, green, and red arrowheads point to lamellipodia, blebs, filipodia, and thick protrusions, respectively. Scale bar: 5 µm. Insets in E and F correspond to a crop with increased intensity for PLCδPHGFP from E and F images, allowing the observation of filipodia structures. Scale bar: 5 µm. (G–I’’) Maximum intensity projection from 10 slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and the genotypes indicated. Egg chambers were stained with aPKC (magenta) and DLG (gray). DNA was visualized by DAPI (blue). Anterior pole of the cluster exhibited in G–G’’ is pointing to the right. White or yellow arrowheads in G’–I’ point to the apical domain (apical cap) of the polar cells or border cells enriched with aPKC. White asterisks in G”–I” indicate non-internalized polar cells. Scale bar: 5 µm. (J–K’’) Maximum intensity projection from four slices (z-step: 0.4 µm) of border cell clusters expressing UAS-PLCδPHGFP and UAS-SRC-CA#1. Egg chambers were stained with aPKC (magenta). DNA was visualized by DAPI (blue). Yellow asterisks in J’, J’’, K’, and K’’ indicate partially internalized polar cells. White arrowheads in J’ and K’ point to the apical domain of the polar cells enriched with aPKC. Scale bar: 5 µm. Polar cells in G–I, J, and K are outlined with a red dashed line.

SRC hyperactivity disrupts border cell morphology and cluster cohesion. (A) Quantifications of the circularity index of individual border cells for the genotypes indicated. Box plots are used to represent the data. Each box plot shows the median (line) with 25th and 75th percentiles (hinges) plus 1.5 × interquartile ranges (whiskers). Dots represent each cell analyzed, and the total number per genotype is indicated on the top of each box plot. Normal distribution was tested using Kolmogorov–Smirnov test. Since data were not normally distributed, a Mann–Whitney U test (one-tailed) was performed between controls and each experimental condition. P values are shown at the top of the graph. (B–F) Maximum intensity projection from eight slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and the genotypes indicated. White, blue, green, and red arrowheads point to lamellipodia, blebs, filipodia, and thick protrusions, respectively. Scale bar: 5 µm. Insets in E and F correspond to a crop with increased intensity for PLCδPHGFP from E and F images, allowing the observation of filipodia structures. Scale bar: 5 µm. (G–I’’) Maximum intensity projection from 10 slices (z-step: 0.5 µm) of border cell clusters expressing UAS-PLCδPHGFP and the genotypes indicated. Egg chambers were stained with aPKC (magenta) and DLG (gray). DNA was visualized by DAPI (blue). Anterior pole of the cluster exhibited in G–G’’ is pointing to the right. White or yellow arrowheads in G’–I’ point to the apical domain (apical cap) of the polar cells or border cells enriched with aPKC. White asterisks in G”–I” indicate non-internalized polar cells. Scale bar: 5 µm. (J–K’’) Maximum intensity projection from four slices (z-step: 0.4 µm) of border cell clusters expressing UAS-PLCδPHGFP and UAS-SRC-CA#1. Egg chambers were stained with aPKC (magenta). DNA was visualized by DAPI (blue). Yellow asterisks in J’, J’’, K’, and K’’ indicate partially internalized polar cells. White arrowheads in J’ and K’ point to the apical domain of the polar cells enriched with aPKC. Scale bar: 5 µm. Polar cells in G–I, J, and K are outlined with a red dashed line.

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