Figure 3.
Rie1 acts independently of the CLN3 pathway to promote meiotic entry. (A and B) Wild type (B47, blue), rie1∆ (B1574, red), cln3∆ (B2729, yellow), and rie1∆ cln3∆ (B2726, orange) strains were induced to sporulate at 30°C. Progression through the meiotic divisions was determined at the indicated times by DAPI staining (A) and progression through pre-meiotic S-phase by flow cytometry (B). Biological replicates = 3 and error bars indicate SEM. (C) Cell size of diploid wild type, rie1∆, cln3∆, and rie1∆ cln3∆ strains was determined by differential interference contrast microscopy measurements after overnight growth in BYTA (pre-meiotic) medium. The mean is indicated by a black bar and statistical significance (*P < 0.05) was determined by two-sided student’s t test.

Rie1 acts independently of the CLN3 pathway to promote meiotic entry. (A and B) Wild type (B47, blue), rie1∆ (B1574, red), cln3∆ (B2729, yellow), and rie1∆ cln3∆ (B2726, orange) strains were induced to sporulate at 30°C. Progression through the meiotic divisions was determined at the indicated times by DAPI staining (A) and progression through pre-meiotic S-phase by flow cytometry (B). Biological replicates = 3 and error bars indicate SEM. (C) Cell size of diploid wild type, rie1∆, cln3∆, and rie1∆ cln3∆ strains was determined by differential interference contrast microscopy measurements after overnight growth in BYTA (pre-meiotic) medium. The mean is indicated by a black bar and statistical significance (*P < 0.05) was determined by two-sided student’s t test.

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