Figure 6.
The ciliary cap complex contains CCDC81. (A) Volcano plot of the peptides identified in FAP256A/B-KO versus WT cilia. The proteins absent in FAP256A/B-KO are listed on the left-hand side of the graph. The proteins only found in FAP256A/B-KO are listed on the right-hand side of the graph. (B) Atomic models of IJ341–225 (red) and the N-terminal domain of CCDC811–223 (green) are shown in Fig. S5 H as well as the two models overlaid. (C) Alignment of Tetrahymena IJ341–225 and CCDC811–223. Residues in white on a red background are conserved, and residues in red have similar properties. The secondary structure of IJ34 is shown above the alignment. (D) Predicted AlphaFold atomic model of the putative microtubule-binding domain CCDC811–223 (light green) along with α-tubulin (green) and β-tubulin (blue). The TOG domain of FAP256426–678 (pink) is shown bound to an adjacent curved tubulin dimer. (E) Representative SR-SIM images of cells expressing CCDC81-GFP-mNeonGreen under the native promoter fixed and labeled with AXO49 anti-polyglycylated (polyG) tubulin monoclonal antibody that labels ciliary axonemes (green), anti-GFP polyclonal antibodies (red), and DAPI (blue). Inset highlights CCDC81 at the tips of locomotory lateral cilia. Scale bars 10 μm, inset 5 μm. Abbreviations: om, oral membranelle; cvp, contractile vacuole pore. Arrowheads point to ciliary tips. (F) Live imaging of CCDC81. Brightfield, TIRF, and merged images of the anterior regions of Tetrahymena cells expressing CCDC81-GFP-mNeonGreen. Short arrows point to signals present in the distal region of moving locomotory cilia. Inset displays a kymograph showing movements of the CCDC81 signal at the distal portion of a locomotory cilium. Red bilateral arrow marks the areas used for the kymograph. The images shown were extracted from Video 2. Scale bar: 10 μm. Horizontal scale bar for kymograph: 2 s, vertical scale bar 2 μm.

The ciliary cap complex contains CCDC81. (A) Volcano plot of the peptides identified in FAP256A/B-KO versus WT cilia. The proteins absent in FAP256A/B-KO are listed on the left-hand side of the graph. The proteins only found in FAP256A/B-KO are listed on the right-hand side of the graph. (B) Atomic models of IJ341–225 (red) and the N-terminal domain of CCDC811–223 (green) are shown in Fig. S5 H as well as the two models overlaid. (C) Alignment of Tetrahymena IJ341–225 and CCDC811–223. Residues in white on a red background are conserved, and residues in red have similar properties. The secondary structure of IJ34 is shown above the alignment. (D) Predicted AlphaFold atomic model of the putative microtubule-binding domain CCDC811–223 (light green) along with α-tubulin (green) and β-tubulin (blue). The TOG domain of FAP256426–678 (pink) is shown bound to an adjacent curved tubulin dimer. (E) Representative SR-SIM images of cells expressing CCDC81-GFP-mNeonGreen under the native promoter fixed and labeled with AXO49 anti-polyglycylated (polyG) tubulin monoclonal antibody that labels ciliary axonemes (green), anti-GFP polyclonal antibodies (red), and DAPI (blue). Inset highlights CCDC81 at the tips of locomotory lateral cilia. Scale bars 10 μm, inset 5 μm. Abbreviations: om, oral membranelle; cvp, contractile vacuole pore. Arrowheads point to ciliary tips. (F) Live imaging of CCDC81. Brightfield, TIRF, and merged images of the anterior regions of Tetrahymena cells expressing CCDC81-GFP-mNeonGreen. Short arrows point to signals present in the distal region of moving locomotory cilia. Inset displays a kymograph showing movements of the CCDC81 signal at the distal portion of a locomotory cilium. Red bilateral arrow marks the areas used for the kymograph. The images shown were extracted from Video 2. Scale bar: 10 μm. Horizontal scale bar for kymograph: 2 s, vertical scale bar 2 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal