The A-tubules are linked to the membrane and to each other in the ciliary tip. (A) 3D model of the ciliary tip with inset showing A-tubule links in green and membrane links in yellow. (B–E) Representative raw tomogram slices along with cross (top) and longitudinal (bottom) slices of subtomogram averages of individual A-tubules. Green arrowheads point to A-tubule links (B and C) and yellow arrowheads point to membrane links (D and E). Scale bars tomograms: 100 nm, subtomogram averages: 25 nm. (F) Graph showing the distance between pairs of A-tubule singlets in the ciliary tip (n = 6 cilia). Standard deviation is shown in light blue.