Figure 7.
Myosin II cortical flashes correspond with nuclear movement and shape changes. (A and B) Example images of Sqh-mCherry in control (A) and Lam-depleted clusters (B) after 1 d at 29°C (see also Fig. S5, F and G, for 3 d at 29°C). Sqh-mCherry aggregates at apical surfaces in polar cells (pc) as previously reported (Mishra et al., 2019b) and has been covered to focus on cortical flashes. Yellow arrowheads point to Sqh flashes. (C) Average number of myosin flashes observed divided by the total number of time frames acquired. RNAi condition: 3 d at 29°C. n = 9 (w), 8 (Lam1), and 8 (Lam2) egg chambers. Statistical test: one-way ANOVA with post-hoc Tukey. (D) Images from a time-lapse series showing myosin flashes around the nucleus. Yellow arrowheads point to flashes. (E and F) Average change in nuclear position along the anterior–posterior axis of the egg chamber (E), and average nuclear aspect ratio change (F) relative to presence and position of myosin flashes. N = 16 movies; each dot represents one nucleus; n = number of nuclei: 22 (no flash), 12 (front), 21 (side), and 16 (back). Bars: mean ± SEM. Statistical tests E and F: one-way ANOVA followed by Tukey post-hoc. (G and H) Images of control and Sqh-depleted clusters showing that nuclei stay behind protrusion necks upon sqhRNAi, 1 d at 29°C. (I) Plot of the change in nuclear aspect ratio of leader cells. Dots: individual nuclei, n = 6 (control) and 13 (sqh). Middle bar and error bars: mean ± SEM. 1 d at 29°C. Each dot represents one nucleus. Statistical test: Mann–Whitney Test. (J and K) Working model for how nuclei and myosin coordinate delamination and invasion into confined space. Genotypes and experimental replicates reported in Table S2. Scale bars: 10 μm.

Myosin II cortical flashes correspond with nuclear movement and shape changes. (A and B) Example images of Sqh-mCherry in control (A) and Lam-depleted clusters (B) after 1 d at 29°C (see also Fig. S5, F and G, for 3 d at 29°C). Sqh-mCherry aggregates at apical surfaces in polar cells (pc) as previously reported (Mishra et al., 2019b) and has been covered to focus on cortical flashes. Yellow arrowheads point to Sqh flashes. (C) Average number of myosin flashes observed divided by the total number of time frames acquired. RNAi condition: 3 d at 29°C. n = 9 (w), 8 (Lam1), and 8 (Lam2) egg chambers. Statistical test: one-way ANOVA with post-hoc Tukey. (D) Images from a time-lapse series showing myosin flashes around the nucleus. Yellow arrowheads point to flashes. (E and F) Average change in nuclear position along the anterior–posterior axis of the egg chamber (E), and average nuclear aspect ratio change (F) relative to presence and position of myosin flashes. N = 16 movies; each dot represents one nucleus; n = number of nuclei: 22 (no flash), 12 (front), 21 (side), and 16 (back). Bars: mean ± SEM. Statistical tests E and F: one-way ANOVA followed by Tukey post-hoc. (G and H) Images of control and Sqh-depleted clusters showing that nuclei stay behind protrusion necks upon sqhRNAi, 1 d at 29°C. (I) Plot of the change in nuclear aspect ratio of leader cells. Dots: individual nuclei, n = 6 (control) and 13 (sqh). Middle bar and error bars: mean ± SEM. 1 d at 29°C. Each dot represents one nucleus. Statistical test: Mann–Whitney Test. (J and K) Working model for how nuclei and myosin coordinate delamination and invasion into confined space. Genotypes and experimental replicates reported in Table S2. Scale bars: 10 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal