Border cells do not require the LINC complex for migration and lack calcium dynamics but have rapid flashes of myosin around nuclei. (A) Migration indices for the indicated conditions. iPLA2 mutants are iPLA2-VIA[Delta174] and iPLA2-VIA[Delta192], which are both predicted to excise the start codon and have been validated as null alleles by antibody staining (Lin et al., 2018). N = 3 experimental replicates. (B and C) Select images of border cells (B) or follicle cells (C) expressing UAS-GCaMP6S driven by c306Gal4. Arrowhead: calcium pulse. (D) Plot showing the individual and mean +/ SEM fluorescent intensity of GCAMP6s. n = 7 (follicle) and n = 6 (border) cells from independent clusters. A two-sided unpaired t test was performed. (E) Plot of the maximum change in border cells and follicle cells with flashes. Individual (dots) and mean ± SEM (bars) are shown. A Welch’s t test was performed. (F and G) Example images of Sqh-mCherry in control (F) and Lam-depleted clusters (G) after 3 d incubation at 29°C. Arrowheads: myosin flashes. (H and I) Example images of border cell clusters stained for Klar and indicated markers in control (H) and klarRNAi (I). (J) Migration index for indicated conditions. Fisher’s exact test with Bonferroni correction to compare percentage with complete migration (wRNAi versus klarRNAi: P = 0.003, wRNAi versus koiRNAi: P > 0.9, and wRNAi versus Msp300RNAi, P > 0.9). Genotypes and experimental replicates reported in Table S2.