There is no detectable diffusion barrier in the leading border cell. (A and B) Images from-time lapses series where a photoactivatable (PA) GFP-ɑtubulin was activated in a border cell (A) or a posterior follicle cell (B). (A′ and B′) Images with regions of interest (ROIs) that were measured. ROIA: activated region (blue box); ROIB: unactivated region (yellow box). Scale bar: 10 μm. Time is relative to the start of the movie and photoactivation occurred at 6 s. (C) Plot showing the mean ± SEM of the ratio of the F.I. of GFP in front:back ROIs over time. Experiments were performed on three different experimental days and n = number of movies is displayed for each condition. (D) Time constants are calculated from the slope of the Boltzmann sigmoidal fitted curve (see Materials and methods). A Kruskal–Wallis test was performed. Genotypes and experimental replicates are reported in Table S2.
