Figure 5.
B-type lamins promote the expansion of a single protrusion. (A and B) Single z-slice images from time-lapse series of migrating control border cells (wRNAi, A) or Lam-depleted border cells that delaminate but fail to expand the protrusion (B). White bars show protrusion width. Inset in B shows the lead cell nucleus with a backward herniation (arrowhead). Scale bars: 10 μm. (C–E) Plots showing average ± SEM (bars) and individual movie values (dots) of the average length (C) and width of protrusions (D and E) for the indicated conditions. n = 16 (w), 8 (Lam1), and 10 (Lam2) border cell clusters. (E) Average protrusion width relative to nuclear movement for each RNAi line; a number of leading cells with indicated condition left to right, n = 12, 16, 12, 6, 8, 6, 8, 10, 8. One-way ANOVAs with Tukey post-hocs were performed for each plot.

B-type lamins promote the expansion of a single protrusion. (A and B) Single z-slice images from time-lapse series of migrating control border cells (wRNAi, A) or Lam-depleted border cells that delaminate but fail to expand the protrusion (B). White bars show protrusion width. Inset in B shows the lead cell nucleus with a backward herniation (arrowhead). Scale bars: 10 μm. (C–E) Plots showing average ± SEM (bars) and individual movie values (dots) of the average length (C) and width of protrusions (D and E) for the indicated conditions. n = 16 (w), 8 (Lam1), and 10 (Lam2) border cell clusters. (E) Average protrusion width relative to nuclear movement for each RNAi line; a number of leading cells with indicated condition left to right, n = 12, 16, 12, 6, 8, 6, 8, 10, 8. One-way ANOVAs with Tukey post-hocs were performed for each plot.

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