Figure 4.
Lamin overexpression compensates for Lam RNAi, but strong Lam overexpression impedes border cell migration. (A–D) Representative images of stage 10 egg chambers with overexpression (OE) of the indicated UAS constructs in border cells by c306Gal4. The white box marks border cell clusters. (E) Migration indices for the indicated conditions. n = number of egg chambers. LacZ is the control. A Fisher’s exact test with a Bonferroni correction to test for proportion with complete migration yields a significant result between LacZ versus Lam OE, P <0.0003. All other comparisons to LacZ are not significant. (F) Plot of cluster speed with individual movies (dots) and mean ± SEM plotted. n = 7 (LacZ), 6 (LamRE), 7 (Lam), and 4 (LamC) egg chambers. Statistical test: one-way ANOVA followed by post-hoc Tukey. (G–J) Representative images of stage 10 egg chambers for the indicated conditions. White box surrounds border cell cluster. (K) Plot of the mean ± SEM percentages of egg chambers with completed migration, each dot represents one experimental replicate (N). n (number of egg chambers): wRNAi/LacZ: 47; LacZ/Lam1RNAi: 290; Lam1RNAi/LamRE: 59; Lam1RNAi/LamOE: 73; LamC/Lam1RNAi: 149. Statistical test: Brown–Forsythe and Welch followed by Dunnett’s Multiple Comparison test. Genotypes and experimental replicates reported in Table S2. Scale bars: 10 μm.

Lamin overexpression compensates for Lam RNAi, but strong Lam overexpression impedes border cell migration. (A–D) Representative images of stage 10 egg chambers with overexpression (OE) of the indicated UAS constructs in border cells by c306Gal4. The white box marks border cell clusters. (E) Migration indices for the indicated conditions. n = number of egg chambers. LacZ is the control. A Fisher’s exact test with a Bonferroni correction to test for proportion with complete migration yields a significant result between LacZ versus Lam OE, P <0.0003. All other comparisons to LacZ are not significant. (F) Plot of cluster speed with individual movies (dots) and mean ± SEM plotted. n = 7 (LacZ), 6 (LamRE), 7 (Lam), and 4 (LamC) egg chambers. Statistical test: one-way ANOVA followed by post-hoc Tukey. (G–J) Representative images of stage 10 egg chambers for the indicated conditions. White box surrounds border cell cluster. (K) Plot of the mean ± SEM percentages of egg chambers with completed migration, each dot represents one experimental replicate (N). n (number of egg chambers): wRNAi/LacZ: 47; LacZ/Lam1RNAi: 290; Lam1RNAi/LamRE: 59; Lam1RNAi/LamOE: 73; LamC/Lam1RNAi: 149. Statistical test: Brown–Forsythe and Welch followed by Dunnett’s Multiple Comparison test. Genotypes and experimental replicates reported in Table S2. Scale bars: 10 μm.

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