Figure 3.
B-type lamins are required to maintain nuclear shape and integrity. (A–C) Images of border cells from time-lapse series in stage 9 egg chambers with the indicated markers and conditions. Yellow arrowheads point to cells with dsRED.NLS throughout the cell. (A–C′) Grayscale images of nuclei (dsRED.NLS). (D) Plot measuring the percentage of cells with dsRED.NLS throughout the cell versus in the nucleus. A Fisher’s exact test with a Bonferroni correction for multiple testing was performed: (w versus Lam1 P = 0.01; w versus Lam2, P = 0.009; w versus LamC, P > 0.9). n = number of nuclei. (E and F) Images of border cells from time-lapse series with the indicated markers and conditions. Yellow arrowhead marks backward nuclear herniation. (E′ and F′) Grayscale images of nuclei (HisRFP). (G) Plot with the percent of nuclei with herniations in each condition. A Fisher’s exact test with a Bonferroni correction for multiple testing was performed: (w versus Lam1 P = 0.001; w versus Lam2,P = 0.0009; w versus LamC, P > 0.9). n = number of nuclei. (H) Plot of individual and mean ± SEM nuclear aspect ratios when the nucleus extended into the protrusion (defined as >1 SD above average nucleus distance to polar cell boundary). n = 8 (w), 3 (Lam1), and 5 (Lam2) leader nuclei. A one-way ANOVA with post-hoc Tukey was performed. (I) Plot of individual and average ± SEM values for nuclear circularity. A Kruskal–Wallis test was performed. n = 15 (w), 13 (LamC), 12(Lam1), and 16 (Lam2) leader cell nuclei. (J and K) Images of fixed border cell clusters stained with indicated markers for wRNAi (J) and Lam2RNAi (K) border cells. (J′ and K′) Grayscale images of DNA (Hoechst). (L and M) Measurements of nuclear area and cell area. Each individual area value shown as dots and bars representing mean ± SEM. Nuclei number (L); n = 290 (w), 201 (Lam1), and 221 (Lam2). Cell number (M), n = 80 (w), 152(Lam1), and 104 (Lam2). Statistical test for L and M: Kruskal–Wallis test. Scale bars: 10 μm. KD conditions: 1 d at 29°C except the cell area (M), which was 3 d at 29°C. Genotypes and experimental replicates reported in Table S2.

B-type lamins are required to maintain nuclear shape and integrity. (A–C) Images of border cells from time-lapse series in stage 9 egg chambers with the indicated markers and conditions. Yellow arrowheads point to cells with dsRED.NLS throughout the cell. (A–C′) Grayscale images of nuclei (dsRED.NLS). (D) Plot measuring the percentage of cells with dsRED.NLS throughout the cell versus in the nucleus. A Fisher’s exact test with a Bonferroni correction for multiple testing was performed: (w versus Lam1 P = 0.01; w versus Lam2, P = 0.009; w versus LamC, P > 0.9). n = number of nuclei. (E and F) Images of border cells from time-lapse series with the indicated markers and conditions. Yellow arrowhead marks backward nuclear herniation. (E′ and F′) Grayscale images of nuclei (HisRFP). (G) Plot with the percent of nuclei with herniations in each condition. A Fisher’s exact test with a Bonferroni correction for multiple testing was performed: (w versus Lam1 P = 0.001; w versus Lam2,P = 0.0009; w versus LamC, P > 0.9). n = number of nuclei. (H) Plot of individual and mean ± SEM nuclear aspect ratios when the nucleus extended into the protrusion (defined as >1 SD above average nucleus distance to polar cell boundary). n = 8 (w), 3 (Lam1), and 5 (Lam2) leader nuclei. A one-way ANOVA with post-hoc Tukey was performed. (I) Plot of individual and average ± SEM values for nuclear circularity. A Kruskal–Wallis test was performed. n = 15 (w), 13 (LamC), 12(Lam1), and 16 (Lam2) leader cell nuclei. (J and K) Images of fixed border cell clusters stained with indicated markers for wRNAi (J) and Lam2RNAi (K) border cells. (J′ and K′) Grayscale images of DNA (Hoechst). (L and M) Measurements of nuclear area and cell area. Each individual area value shown as dots and bars representing mean ± SEM. Nuclei number (L); n = 290 (w), 201 (Lam1), and 221 (Lam2). Cell number (M), n = 80 (w), 152(Lam1), and 104 (Lam2). Statistical test for L and M: Kruskal–Wallis test. Scale bars: 10 μm. KD conditions: 1 d at 29°C except the cell area (M), which was 3 d at 29°C. Genotypes and experimental replicates reported in Table S2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal