Figure 8.
CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Deconvoluted confocal images of immunofluorescence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5-mScarlet-I undergoing nuclear envelop reformation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nuclear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Deconvoluted confocal images of immunofluorescence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5-mScarlet-I undergoing nuclear envelop reformation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nuclear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal