Figure S5.
Vps4-triggered depolymerization of Did2 and Ist1 from Vps60-based ESCRT-III filaments. (A) Quantification of Did2 intensity and kymographs of time-lapse experiments of the addition of Vps4/ATP or Ist1 and Vps4/ATP to SLBs preincubated with Vps60, Vps2, Vps24, Alexa488-Did2, and Ist1. Related to Fig. 5 E (−Ist1 condition) and panel C (+Ist1 corresponds to −Vta1 experiment). (B) Quantification of Ist1 intensity and kymographs of time-lapse experiments of addition of Vps4/ATP or Ist1 and Vps4/ATP to SLBs preincubated with Vps60, Vps2, Vps24, Did2, and Alexa488-Ist1. Related to panel D (+Ist1 corresponds to −Vta1 experiment). (C) Quantification of Did2 intensity and kymographs of time-lapse experiments of the addition of Ist1 and Vps4/ATP or Ist1, Vps4/ATP, or Vta1 to SLBs preincubated with Vps60, Vps2, Vps24, Alexa488-Did2, and Ist1. Related to A (+Ist1 corresponds to −Vta1 experiment). (D) Quantification of Ist1 intensity and kymographs of time-lapse experiments of addition of Ist1 and Vps4/ATP or Ist1, Vps4/ATP, and Vta1 to SLBs preincubated with Vps60, Vps2, Vps24, Did2, and Alexa488-Ist1. Related to B (+Ist1 corresponds to −Vta1 experiment). (E) Cartoon of the model for interplay of Vps4, Did2, and Ist1 during disassembly of ESCRT-III polymers in presence or absence of Vta1. (F) Single-channel confocal images of the experiment shown in Fig. 5. (G) Single-channel confocal images of the experiment shown in Fig. 5. (H) Quantification of Vps2 fluorescence intensities total membrane coverage and colocalization with Snf7 patches from experiments described in Fig. 5, H and I (n ≥ 3). Scale bar: 10 μm.

Vps4-triggered depolymerization of Did2 and Ist1 from Vps60-based ESCRT-III filaments. (A) Quantification of Did2 intensity and kymographs of time-lapse experiments of the addition of Vps4/ATP or Ist1 and Vps4/ATP to SLBs preincubated with Vps60, Vps2, Vps24, Alexa488-Did2, and Ist1. Related to Fig. 5 E (−Ist1 condition) and panel C (+Ist1 corresponds to −Vta1 experiment). (B) Quantification of Ist1 intensity and kymographs of time-lapse experiments of addition of Vps4/ATP or Ist1 and Vps4/ATP to SLBs preincubated with Vps60, Vps2, Vps24, Did2, and Alexa488-Ist1. Related to panel D (+Ist1 corresponds to −Vta1 experiment). (C) Quantification of Did2 intensity and kymographs of time-lapse experiments of the addition of Ist1 and Vps4/ATP or Ist1, Vps4/ATP, or Vta1 to SLBs preincubated with Vps60, Vps2, Vps24, Alexa488-Did2, and Ist1. Related to A (+Ist1 corresponds to −Vta1 experiment). (D) Quantification of Ist1 intensity and kymographs of time-lapse experiments of addition of Ist1 and Vps4/ATP or Ist1, Vps4/ATP, and Vta1 to SLBs preincubated with Vps60, Vps2, Vps24, Did2, and Alexa488-Ist1. Related to B (+Ist1 corresponds to −Vta1 experiment). (E) Cartoon of the model for interplay of Vps4, Did2, and Ist1 during disassembly of ESCRT-III polymers in presence or absence of Vta1. (F) Single-channel confocal images of the experiment shown in Fig. 5. (G) Single-channel confocal images of the experiment shown in Fig. 5. (H) Quantification of Vps2 fluorescence intensities total membrane coverage and colocalization with Snf7 patches from experiments described in Fig. 5, H and I (n ≥ 3). Scale bar: 10 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal