Figure 4.
![Vps60 triggers ESCRT-III copolymer formation. (A–C) Negative stain electron micrographs of Vps60 filaments incubated with indicated proteins polymerized on LUVs (scale bar: 100 nm), representative profile scan of filaments, and schematic representation of observed polymers. (D) Quantification of filament width from experiments described in A–C (n = 3, ROI = 285 [Vps60]; ROI = 56 [Vps60–Vps2–Vps24]; ROI = 21 [Vps60–Vps2–Vp24–Did2]). (E) Negative stain electron micrographs of Vps60-Vps2-Vps24. Scale bar: 100 μm.](https://cdn.rupress.org/rup/content_public/journal/jcb/222/11/10.1083_jcb.202206028/2/jcb_202206028_fig4.png?Expires=1773807560&Signature=V5C1lVB96XqkPQC~sIJ-og0SRCv9JKfKohuOBvJKdJHj1~tdvU9QL7SegacwGNX95bz865bQJSO9xZeNUyaV4rzKSf3eYGP~hBFN3jL6-~uzpde82dvjM16ShKloBBeRLuR9YLUq48BebbP8PYosSP86yzglwCDBb56MZ8d5eInvGDsc1dWXZMSBydLaSqJM~Uh9KJ-R5GcuIw7ld173nt~-jPEzzvFEkivJ5UXzACOzV96owPam86SWlHKIW40pfCVo8ziG6DE5QGjFOfz4ULTrlf-WwnQB0p0LYDhz8qGfoAqAJw-k~rRTjb4UUrVueFPG3r8Zk9fW7tmX-UGUqw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Vps60 triggers ESCRT-III copolymer formation. (A–C) Negative stain electron micrographs of Vps60 filaments incubated with indicated proteins polymerized on LUVs (scale bar: 100 nm), representative profile scan of filaments, and schematic representation of observed polymers. (D) Quantification of filament width from experiments described in A–C (n = 3, ROI = 285 [Vps60]; ROI = 56 [Vps60–Vps2–Vps24]; ROI = 21 [Vps60–Vps2–Vp24–Did2]). (E) Negative stain electron micrographs of Vps60-Vps2-Vps24. Scale bar: 100 μm.
