Figure S4.
Membrane-bound Vps60 recruits ESCRT-III proteins. (A) Confocal images of experiments described in Fig. 3 E. (B and C) Quantification of Did2 intensity (B n = 3, −Vta1 ROI = 24, +Vta1 ROI = 25) and confocal images (C) of Vps60-coverd SLBs incubated with Vps2, Vps24, Alexa488-Did2, and Ist1 in presence or absence of Vta1. (D) Confocal images of experiments described in Fig. 3 F. Scale bar: 10 μm.

Membrane-bound Vps60 recruits ESCRT-III proteins. (A) Confocal images of experiments described in Fig. 3 E. (B and C) Quantification of Did2 intensity (B n = 3, −Vta1 ROI = 24, +Vta1 ROI = 25) and confocal images (C) of Vps60-coverd SLBs incubated with Vps2, Vps24, Alexa488-Did2, and Ist1 in presence or absence of Vta1. (D) Confocal images of experiments described in Fig. 3 F. Scale bar: 10 μm.

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