Figure S3.
Vps60 and Snf7 bind membrane independently from each other. (A) Confocal images of experiment described in Fig. 2 H (scale bar: 10 μm). (B) Confocal images of the time-lapse experiment of addition of Vps60 (red) to SLBs (blue) preincubated with Snf7 (green), Vps2, Vps24, and Did2 (n = 3; scale bar: 2 μm). (C and D) Confocal images and average time projection of time-lapse experiments of the addition of Atto565-Vps60 and Vps4/ATP (C) or Vps24, Vps2, Did2, Ist1, and Vps4/ATP (D; n ≥ 3, ROI ≥ 12; scale bar: 5 μm). (E and F) Negative stain electron micrographs of Vps60 filaments incubated with indicated proteins polymerized on LUVs (scale bar: 100 nm). (G) Negative stain electron micrographs of Vps60 filaments incubated Vps2 and Vps24 (scale bar: 100 nm).

Vps60 and Snf7 bind membrane independently from each other. (A) Confocal images of experiment described in Fig. 2 H (scale bar: 10 μm). (B) Confocal images of the time-lapse experiment of addition of Vps60 (red) to SLBs (blue) preincubated with Snf7 (green), Vps2, Vps24, and Did2 (n = 3; scale bar: 2 μm). (C and D) Confocal images and average time projection of time-lapse experiments of the addition of Atto565-Vps60 and Vps4/ATP (C) or Vps24, Vps2, Did2, Ist1, and Vps4/ATP (D; n ≥ 3, ROI ≥ 12; scale bar: 5 μm). (E and F) Negative stain electron micrographs of Vps60 filaments incubated with indicated proteins polymerized on LUVs (scale bar: 100 nm). (G) Negative stain electron micrographs of Vps60 filaments incubated Vps2 and Vps24 (scale bar: 100 nm).

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