Figure S2.
Vps60 nucleation pattern on SLBs. (A) Confocal images of time-lapse experiments of SLBs incubated with the indicated mixture of proteins (scale bar: 10 μm) described in Fig. 2 D. (B) Quantification of Vps60 intensity from experiments described in Fig. 2 A (n ≥ 3, Vps60 ROI = 13, Snf7_Vps60 ROI = 8, Snf7+Vps60 ROI = 6; scale bar: 10 μm). (C) Confocal images of time-lapse experiments of the addition of Alexa488-Snf7 to Vps60-covered SLBs (scale bar: 10 μm). (D) Confocal images of experiments described in Fig. 2 F (scale bar: 2 μm). (E and F) Quantification of Snf7 intensity (E) and confocal images (F) of time-lapse experiments of addition of 750 nM Vps60 (unlabeled) to SLBs with pregrown Snf7 patches (scale bar: 5 μm). (G and H) Quantification of Vps60 intensity (G) and confocal images (H) of time-lapse experiments of the addition of indicated concentrations of Snf7 (unlabeled) to SLBs with preincubated with Atto565-Vps60 (scale bar: 2 μm).

Vps60 nucleation pattern on SLBs. (A) Confocal images of time-lapse experiments of SLBs incubated with the indicated mixture of proteins (scale bar: 10 μm) described in Fig. 2 D. (B) Quantification of Vps60 intensity from experiments described in Fig. 2 A (n ≥ 3, Vps60 ROI = 13, Snf7_Vps60 ROI = 8, Snf7+Vps60 ROI = 6; scale bar: 10 μm). (C) Confocal images of time-lapse experiments of the addition of Alexa488-Snf7 to Vps60-covered SLBs (scale bar: 10 μm). (D) Confocal images of experiments described in Fig. 2 F (scale bar: 2 μm). (E and F) Quantification of Snf7 intensity (E) and confocal images (F) of time-lapse experiments of addition of 750 nM Vps60 (unlabeled) to SLBs with pregrown Snf7 patches (scale bar: 5 μm). (G and H) Quantification of Vps60 intensity (G) and confocal images (H) of time-lapse experiments of the addition of indicated concentrations of Snf7 (unlabeled) to SLBs with preincubated with Atto565-Vps60 (scale bar: 2 μm).

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