Figure S1.
Characterization of ESCRT-III protein properties. (A) Coomassie gel of purified Vps60. (B) Single-channel confocal microscopy images of the experiment described in Fig. 1 A. Snf7-Alexa488 (green), membrane nanotubes (red); scale bar: 5 μm. (C) Confocal microscopy images of Vps60 (green) binding to membrane nanotubes (red; scale bar: 5 μm). (D) Confocal microscopy images of Vps2-Alex488 (green) and Vps24 binding to membrane nanotubes (red; scale bar: 5 μm). (E) Confocal microscopy images of Vps2-Alex488 (green), Did2-Atto565 (red), and Ist1 binding to membrane nanotubes (blue; scale bar: 5 μm). (F) Confocal images of (Vps2-Alexa488)-Vps24 (green) or (Did2-Alexa488)-Vps2-Ist1 (green) nucleation on SLBs (red; scale bar: 10 μm). (G) Quantification and kymographs of dynamics of Snf7 (A), Vps60 (B), Vps2-Vps24 (C), or Vps2-Did2-Ist1 (D) binding to membrane at indicated pH. Source data are available for this figure: SourceData FS1.

Characterization of ESCRT-III protein properties. (A) Coomassie gel of purified Vps60. (B) Single-channel confocal microscopy images of the experiment described in Fig. 1 A. Snf7-Alexa488 (green), membrane nanotubes (red); scale bar: 5 μm. (C) Confocal microscopy images of Vps60 (green) binding to membrane nanotubes (red; scale bar: 5 μm). (D) Confocal microscopy images of Vps2-Alex488 (green) and Vps24 binding to membrane nanotubes (red; scale bar: 5 μm). (E) Confocal microscopy images of Vps2-Alex488 (green), Did2-Atto565 (red), and Ist1 binding to membrane nanotubes (blue; scale bar: 5 μm). (F) Confocal images of (Vps2-Alexa488)-Vps24 (green) or (Did2-Alexa488)-Vps2-Ist1 (green) nucleation on SLBs (red; scale bar: 10 μm). (G) Quantification and kymographs of dynamics of Snf7 (A), Vps60 (B), Vps2-Vps24 (C), or Vps2-Did2-Ist1 (D) binding to membrane at indicated pH. Source data are available for this figure: SourceData FS1.

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