Depolymerization of non-polarity-marked and polarity-marked GMPCPP microtubules by KIF2A. (A) Ratio of intensities of mScarlet-KIF2A at the fast and slowly growing ends of non-polarity-marked microtubules to its intensity on the microtubule lattice (see Materials and methods). Measurements made from n = 27 microtubules. Error bars are SEM. (B) Example kymograph demonstrating the minus-end selectivity of KIF2A depolymerzing a polarity-marked GMPCPP microtubule. Polarity as indicated. (C) Depolymerization speeds of minus and plus ends of polarity-marked microtubules in the presence of 20 nM untagged KIF2A. Measurements were made from n = 69 microtubules. Error bars are SEM.