Figure 4.
Mdi1 plays a functionally conserved role in mitochondrial division. (A) CLIME analysis (Li et al., 2014) of Mdi1 identifies conservation among fungal, but not plant or metazoan, species. (B) Maximum intensity projections of deconvolved fluorescence microscopy images of the indicated S. cerevisiae strains expressing mito-dsRed and, where indicated, chromosomally integrated S. pombe Mdi1 (SpMdi1) driven by the S. cerevisiae MDI1 promoter. (C) A graph depicting the categorization of mitochondrial morphology of cells as in B. Data shown represent ∼100 cells per strain in each of the three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represents unpaired two-tailed t test. (D) Single-plane deconvolved fluorescence microscopy images of the indicated S. pombe strains expressing the matrix marker mito-mCherry. (E) A graph depicting the categorization of mitochondrial morphology of cells as in D. Data shown represent ∼100 cells per strain in each of three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (F) Single plane (left) and maximum intensity projection (right) deconvolved fluorescence microscopy images of wild-type (top) and ∆mdi1 (bottom) fission yeast cells expressing chromosomally tagged Tom20-mCherry (magenta) and Dnm1-EGFP (green). Arrows mark sites of Dnm1 recruitment to mitochondria. (G) As in F for cells treated for 30 min with 25 µM CCCP. Cell boundaries are indicated with dotted lines. Scale bars = 3 µm (B), 4 µm (D), 5 µm (F and G).

Mdi1 plays a functionally conserved role in mitochondrial division. (A) CLIME analysis (Li et al., 2014) of Mdi1 identifies conservation among fungal, but not plant or metazoan, species. (B) Maximum intensity projections of deconvolved fluorescence microscopy images of the indicated S. cerevisiae strains expressing mito-dsRed and, where indicated, chromosomally integrated S. pombe Mdi1 (SpMdi1) driven by the S. cerevisiae MDI1 promoter. (C) A graph depicting the categorization of mitochondrial morphology of cells as in B. Data shown represent ∼100 cells per strain in each of the three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represents unpaired two-tailed t test. (D) Single-plane deconvolved fluorescence microscopy images of the indicated S. pombe strains expressing the matrix marker mito-mCherry. (E) A graph depicting the categorization of mitochondrial morphology of cells as in D. Data shown represent ∼100 cells per strain in each of three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (F) Single plane (left) and maximum intensity projection (right) deconvolved fluorescence microscopy images of wild-type (top) and ∆mdi1 (bottom) fission yeast cells expressing chromosomally tagged Tom20-mCherry (magenta) and Dnm1-EGFP (green). Arrows mark sites of Dnm1 recruitment to mitochondria. (G) As in F for cells treated for 30 min with 25 µM CCCP. Cell boundaries are indicated with dotted lines. Scale bars = 3 µm (B), 4 µm (D), 5 µm (F and G).

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