Mdi1 is required for CCCP-induced mitochondrial division. (A) Maximum intensity projections of deconvolved images of the indicated yeast strains expressing mito-dsRed before (top) or after (bottom) treatment with 25 µM CCCP for 45 min. Arrows mark mitochondrial hyper-constrictions that occur after CCCP treatment. (B) A graph depicting the categorization of mitochondrial morphology of cells as in A. Data shown represent at least 75 cells per strain in each of the three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. Cell boundaries are indicated with dotted lines. Scale bar = 3 µm.