Figure 1.
Mitochondrial division is perturbed in the absence of Mdi1. (A) Representative single-plane deconvolved epifluorescence microscopy images of the indicated budding yeast strains expressing the mitochondrial matrix marker mito-dsRed. (B) A graph of the categorization of mitochondrial morphology from cells as in A. Data shown represent at least 75 cells per strain in each of the three independent experiments, and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (C) Maximum intensity projections of epifluorescence microscopy images of the indicated strains expressing mito-dsRed that were grown to log phase at room temperature and imaged before (top) and after (bottom) growth at 37°C for 20 min. (D) A graph depicting the categorization of mitochondrial morphology as in C. Data shown represent at least 70 cells per strain in each of the three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (E) Serial dilutions of the indicated yeast cells were plated on media containing the non-fermentable carbon source ethanol/glycerol (YPEG) and grown at the indicated temperatures. Cell boundaries are indicated with dotted lines. Scale bars = 2 µm (A), 3 µm (C). See also Fig. S1.

Mitochondrial division is perturbed in the absence of Mdi1. (A) Representative single-plane deconvolved epifluorescence microscopy images of the indicated budding yeast strains expressing the mitochondrial matrix marker mito-dsRed. (B) A graph of the categorization of mitochondrial morphology from cells as in A. Data shown represent at least 75 cells per strain in each of the three independent experiments, and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (C) Maximum intensity projections of epifluorescence microscopy images of the indicated strains expressing mito-dsRed that were grown to log phase at room temperature and imaged before (top) and after (bottom) growth at 37°C for 20 min. (D) A graph depicting the categorization of mitochondrial morphology as in C. Data shown represent at least 70 cells per strain in each of the three independent experiments and bars indicate SEM. Asterisks (***P < 0.001) represent unpaired two-tailed t tests. (E) Serial dilutions of the indicated yeast cells were plated on media containing the non-fermentable carbon source ethanol/glycerol (YPEG) and grown at the indicated temperatures. Cell boundaries are indicated with dotted lines. Scale bars = 2 µm (A), 3 µm (C). See also Fig. S1.

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