Anisotropy histograms of simulated clusters. (A–D) Histograms of observed cluster anisotropies (lavender) and clusters simulated with specific distances between the Rtn4 monomers (gray). (E) Anisotropy distributions of observed clusters (lavender), linear-shaped clusters using the radius of gyration (Rg) of observed clusters (green), and linear-shaped clusters using Rg * 0.925 (yellow).