Western blots of CRISPR cell lines. (A–C) Probing U-2 OS Rtn4-Halo and control cell lines with anti-Halo, anti-Rtn4, and anti-α-tubulin antibodies, respectively. (D–F) Probing U-2 OS Rtn4-mEGFP and control cell lines with anti-GFP, anti-Rtn4, and anti-α-tubulin antibodies, respectively. (B and E) Line plots drawn through the U-2 OS Rtn4-Halo and Rtn4-mEGFP bands of tagged (top band/left peak) and untagged Rtn4 (bottom band/right peak), respectively. (G) Histogram of average Sec61β pixel intensity measured along tubules. M: Marker; WT: U-2 OS cells; KO: U-2 OS Rtn4 knock-out cells; C-H: Rtn4-Halo CRISPR clone cells; C-G: Rtn4-mEGFP CRISPR clone cells. Source data are available for this figure: SourceData FS1.