Figure S5.
Masking exposed hydrophobic region of TCRβ in preTCR increases surface expression in T cell line. (A) TCR-negative Jurkats (αβTCR−ve) were lentivirally transduced with the indicated preTCR construct described in main text, and receptor surface expression, as measured by CD3 staining, was compared to wildtype Jurkat T cells (αβTCR). (B) Equivalent data as in A but now showing receptor surface expression levels as a function of preTCR variant expression (mScarlet fluorophore). (C) Quantification of CD137 expression on the three Jurkat T cell variants shown in Fig. 8. Error bars show mean ± SEM (n = 3). (D) Equivalent quantification as in C for PD1 expression on the three Jurkat variants. Error bars show mean ± SEM (n = 3); asterisks indicate P < 0.05 (*) or P < 0.01 (**) when comparing indicated datasets. A two-tailed, two-sided t test was used for all statistical analyses.

Masking exposed hydrophobic region of TCRβ in preTCR increases surface expression in T cell line. (A) TCR-negative Jurkats (αβTCR−ve) were lentivirally transduced with the indicated preTCR construct described in main text, and receptor surface expression, as measured by CD3 staining, was compared to wildtype Jurkat T cells (αβTCR). (B) Equivalent data as in A but now showing receptor surface expression levels as a function of preTCR variant expression (mScarlet fluorophore). (C) Quantification of CD137 expression on the three Jurkat T cell variants shown in Fig. 8. Error bars show mean ± SEM (n = 3). (D) Equivalent quantification as in C for PD1 expression on the three Jurkat variants. Error bars show mean ± SEM (n = 3); asterisks indicate P < 0.05 (*) or P < 0.01 (**) when comparing indicated datasets. A two-tailed, two-sided t test was used for all statistical analyses.

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