Spindles form less robustly in cells depleted of Msps in addition to Cnn, Grip71, and Grip163 ^GCP6 . (A–C) Parts of a whole graphs (A and C) and images (B) represent analyses of mitotic spindle quality in cells fixed and immunostained for alpha-tubulin (microtubules, green), mitotic DNA (blue), and Asl (centrioles, magenta) after 30 s of warming post cooling from either wild-type, cnn,grip71,grip163, or cnn,grip71,grip163,msps mutants, as indicated. N numbers are indicated, and each N represents a single cell. Only cells that had centrosomes and that had already formed a spindle were analyzed in A and B, and only cells lacking centrosomes but that had formed a spindle were analyzed in C. Note that wild-type cells always contained centrosomes and so were not analyzed in C. Note also how spindle quality is frequently low in cnn,grip71,grip163,msps mutants (A and B), but the difference in spindle quality between mutant types is not apparent in cells lacking centrosomes (C). Note also that some cells lacking Cnn have abnormal numbers of centrosomes due to centrosome segregation problems during cell division (Conduit et al., 2010).