Plots of individual centrosome Jup-mCherry values during cooling–warming experiments. (A and B) Graphs plotting the centrosomal signal (after subtraction of cytosolic signal) of Jupiter-mCherry within living Drosophila control (A) and cnn,grip71,grip163 mutant (B) third instar larval brain cells as they were cooled to 5°C for ∼3 min and then rapidly warmed to 20°C. Time in seconds relative to the initiation of warming (0 s) is indicated. Note how the centrosomal Jupiter-mCherry signal does not always reach cytosolic levels (i.e., 0), indicating that microtubules were not fully depolymerized from all centrosomes, but note also that even when the Jupiter-mCherry signal did reach cytosolic levels there was still a rapid increase after warming, indicating that the increase in signal after warming is not simply due to regrowth of partially depolymerized microtubules.